Description:
Summary:
The National Cancer Institute (NCI) seeks licensees for a human T-cell line, C8166-45, transformed by HTLV-1. C8166-45, also known as C63/CRII-2, contains three transcriptionally active proviruses useful for testing biological activities involved in T-cell immortalization and growth.
Description of Technology:
Human T-cell leukemia virus type 1 (HTLV-1) was the first human retrovirus reported and is recognized as an etiological agent of adult T-cell leukemia (ATL). However, only a small percentage of individuals develop symptomatic ATL which carries a poor prognosis. The latency period can last for decades and universal screening for HTLV-1 infection has ceased. Thus, the C8166-45 cell line is a necessary component for understanding the mechanisms of HTLV-1 infection and improving clinical outcomes.
NCI researchers derived C8166-45 by cocultivation or fusion of umbilical cord blood lymphocyte with T-cells cultures from leukemia-lymphoma patients. It is highly permissive to HIV-1 infection and characterized for its suitability in replication-competent lentiviral (RCL) assays to assess its safety for gene therapy products, such as lentiviral vectors. This cell line is highly useful in studying viral protein interactions, immortalization of human T-cells, and HIV replication.
NCI is seeking parties to non-exclusively license the C8166-45 cell line.
Potential Commercial:
- Investigation of HTLV pathogenesis and replication
- Studies of virus-induced T-cell transformation
- Studies of HTLV expression regulation by human T-cells
- Studies of HIV replication
Competitive Advantages:
- Contains a low amount of viral proteins
- Does not release detectable virus particles
- Suitable for testing RCL assay sensitivity