This technology involves neural stem cells (NSCs) derived from pluripotent stem cells (PSCs) that can differentiate into neurons and glia. The key feature of this technology is the CY2 EEF1A1 GFP iPSC line, which includes a green fluorescent protein (GFP) expressed under the EEF1A1 promoter, leading to its ubiquitous expression in cells. This characteristic makes the NSCs and the neural cells differentiated from this line exhibit green fluorescence. Such cells, when transplanted into animal models like mice and rats, can be easily tracked due to their fluorescence. This tracking is critical for assessing whether these cells have successfully integrated into the host's nervous system and are functioning properly. The primary advantage of this technology is that the GFP expression allows for easier tracking of the transplanted cells in comparison to most other NSC lines which lack such fluorescent reporters. This technology has potential applications in studying the integration and functionality of transplanted neurons and astrocytes in animal models, providing significant insights into neural cell behavior and treatment efficacy.