Search Results - mahendra+rao

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Generation of AAVS1 and C13 “Safe Harbor” Transcription Activator-life Effector Nucleases (TALENs) for Drug Screening or Gene Therapy Development

This technology includes AAVS1 and C13 “safe harbor” transcription activator-life effector nucleases (TALENs) for drug screening or gene therapy applications. TALENs are engineered sequence-specific DNA endonucleases that can significantly enhance genome-editing efficiency by >100-1000 folds. “Safe harbor” such as AAVS1 safe...

Published: 9/10/2024 | Inventor(s): Jizhong Zou, Mahendra Rao

Keywords(s):

Category(s): Application > Diagnostics, Application > Research Materials, Application > Therapeutics, Collaboration Sought > Licensing, ResearchProducts > Plasmids/Vectors

Generation of Safe-harbor Gene Targeting Donor Plasmids

This technology includes two safe harbor gene targeting donors, specifically designed for applications in the study of induced pluripotent stem cells (iPSC). These include the pAAVS1D-CMV.RFP-EF1a.copGFPpuro and pAAVS1-iCLHN donors. A key feature of these donors is their ability to integrate various transgenes into specific loci through homologous recombination,...

Published: 9/10/2024 | Inventor(s): Jizhong Zou, Mahendra Rao

Keywords(s):

Category(s): Application > Research Materials, Collaboration Sought > Materials Available, ResearchProducts > Human iPSC Lines, ResearchProducts > Plasmids/Vectors

Generation of Gene Specific Targeting Donor Plasmid (p5KI-GP-CHN and p3KI-GP-CHN)

This technology includes the use of engineered human induced pluripotent stem cells (iPSCs) for various applications such as studying cell differentiation, drug screening, and gene transfer therapy. It employs gene targeting donors flanked by DNA sequences compatible with endogenous loci to integrate transgenes through homologous recombination. A key...

Published: 9/10/2024 | Inventor(s): Jizhong Zou, Mahendra Rao

Keywords(s):

Category(s): Application > Research Materials, Collaboration Sought > Licensing, ResearchProducts > Human iPSC Lines, TherapeuticArea > Neurology

Astrocyte Differentiation of Neural Stem Cells with StemPro Embryonic Stem Cell Serum Free Medium for Research and Potential Therapeutic Use

This technology includes an innovative method for differentiating astrocytes from neural stem cells (NSCs). The process involves using Life Technologies StemPro embryonic stem cell serum-free medium to initially guide NSCs towards a neuronal lineage. Over a period of 28-35 days, as the cells are continually passaged, neurons gradually die off, leading...

Published: 9/10/2024 | Inventor(s): Nasir Malik, Mahendra Rao, Xianmin Zeng

Keywords(s):

Category(s): Application > Research Materials, Collaboration Sought > Materials Available, ResearchProducts > Human Cell Lines, TherapeuticArea > Neurology

Neural Stem Cells from an iPSC Line Ubiquitously Expressing Green Fluorescent Protein for Basic Science Research and Cell Line Tracking

This technology involves neural stem cells (NSCs) derived from pluripotent stem cells (PSCs) that can differentiate into neurons and glia. The key feature of this technology is the CY2 EEF1A1 GFP iPSC line, which includes a green fluorescent protein (GFP) expressed under the EEF1A1 promoter, leading to its ubiquitous expression in cells. This characteristic...

Published: 9/10/2024 | Inventor(s): Nasir Malik, Mahendra Rao

Keywords(s):

Category(s): Application > Research Materials, Collaboration Sought > Materials Available, ResearchProducts > Human Cell Lines, TherapeuticArea > Neurology

Neuronal Differentiation of Neural Stem Cells with StemPro Embryonic Stem Cell Serum Free Medium for Research and Therapeutic Development

This technology involves an innovative method for differentiating neural stem cells (NSCs) into neurons, primarily for use in basic science research and in developing therapies for brain and spinal cord disorders. Existing methods for generating neurons from NSCs typically result in high efficiency but low survival rates, especially when neurons are...

Published: 8/12/2024 | Inventor(s): Nasir Malik, Mahendra Rao

Keywords(s):

Category(s): Application > Research Materials, Collaboration Sought > Licensing, ResearchProducts > Human iPSC Lines, TherapeuticArea > Neurology

Three Cell Lines of Motor Neuron Progenitors for Various Diagnostic and Therapeutic Uses

This technology includes three distinct cell lines of motor neuron progenitors, derived from different sources: human induced pluripotent stem cell (iPSC) line BC1, human iPSC line X1, and human embryonic stem cell (hESC) line H14. These cell lines hold significant potential for multiple diagnostic and therapeutic applications. A key advantage of these...

Published: 8/12/2024 | Inventor(s): Mahendra Rao

Keywords(s):

Category(s): Application > Diagnostics, Collaboration Sought > Materials Available, ResearchProducts > Human iPSC Lines, Application > Therapeutics

Cell Lines of Dopaminergic Neurons Derived from Human Induced Pluripotent Stem Cell (iPSC) lines for Multiple Neurological Therapeutic and Diagnostic Uses

This technology includes three cell lines of dopaminergic neurons derived from human induced pluripotent stem cell (iPSC) line BC1, human iPSG line X1 and human embryonic stem cell (hESC) line H14 to be utilized in neurology research. These cell lines will be used for to study the biology of brain development and may also be used to test different characterization...

Published: 8/12/2024 | Inventor(s): Mahendra Rao

Keywords(s):

Category(s): Application > Research Materials, Collaboration Sought > Licensing, ResearchProducts > Human iPSC Lines, TherapeuticArea > Neurology

Engineered Human Induced Pluripotent Stell Cell (iPSC) Lines for Multiple Therapeutic and Diagnostic Uses

This technology includes ten engineered human induced pluripotent stem cell (iPSC) lines with reported genes inserted into safe harbor sites for use in therapy and diagnostic screening assay development as well as basic stem cell biology research. These cell lines have the potential to differentiate into all cells in the body, and theoretically can...

Published: 9/10/2024 | Inventor(s): Mahendra Rao

Keywords(s):

Category(s): Application > Diagnostics, Application > Research Materials, Application > Therapeutics, Collaboration Sought > Licensing, ResearchProducts > Human iPSC Lines

Engineering Neural Stem Cells Using Homologous Recombination

Methods for modifying the genome of a Neural Stem Cell (NSC) are disclosed. Also, methods for differentiating NSCs into neurons and glia are described. NSCs are multipotent, self-renewing cells found in the central nervous system, capable of differentiating into neurons and glia. NSCs can be generated efficiently from pluripotent stem cells (PSCs)...

Published: 7/30/2024 | Inventor(s): Mahendra Rao, Jizhong Zou, Raymond Funahashi, Nasir Malik

Keywords(s): Cells, EFFECTOR, Gene, Listed LPM Vepa as of 4/15/2015, NB1XXX, Nucleases, Post LPM Assignment Set 20150420, Pre LPM working set 20150418, SOMATIC, TAl, Targeting, VEXXXX, WIXXXX, WJXXXX, XEXXXX, YAXXXX, YBXXXX

Category(s): Collaboration Sought > Licensing, Application > Research Materials, Application > Therapeutics, TherapeuticArea > Neurology

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