This technology includes three distinct cell lines of motor neuron progenitors, derived from different sources: human induced pluripotent stem cell (iPSC) line BC1, human iPSC line X1, and human embryonic stem cell (hESC) line H14. These cell lines hold significant potential for multiple diagnostic and therapeutic applications. A key advantage of these cell lines is the commercial availability of their starting materials (iPSC-BC1, iPSC-X1, and hESC-H14), which are not restricted in terms of usage of their derivatives. This aspect is particularly beneficial as it can considerably reduce the financial burden associated with initiating various research projects. In terms of applications, these cell lines are poised for use in studying brain development biology. They can also play a crucial role in testing different characterization and differentiation assays. Furthermore, the motor neuron progenitors and their derivatives may serve as controls in research, especially in screening for small molecules that can alter cell fate or mitigate the symptoms of various diseases.