Rapid Detection of Multi-Drug-Resistant Mycobacterium tuberculosis Using Real-Time PCR and High-Resolution Melt Analysis

Description:
CDC scientists have developed a rapid, sensitive, and specific real-time PCR assay that is capable of detecting the presence of Mycobacterium tuberculosis and determining its resistance profile to antibiotics, such as rifampicin and isoniazid. Currently, there are few assays available that are capable of both detecting M. tuberculosis and determining the bacteria's drug resistance. This assay incorporates multiple fluorescent chemistries, providing a simple and cost-effective method of determining the bacteria's drug resistance. Additionally, this assay may be used to quickly discriminate Mycobacterium tuberculosis complex (MTBC) strains from non-MTBC strains.
Patent Information:
For Information, Contact:
Jeremiah Mitzelfelt
NIH Technology Transfer
301-443-8518
jeremiah.mitzelfelt@nih.gov
Inventors:
Jonas Winchell
Kelley Cowart
Melissa Ramirez
James Posey
Keywords:
analysis
DA3XXX
DAXXXX
Detection
DXXXXX
High
Melt
Multidrug
PCR
PROCESS
REAL-TIME
RESISTANT
RESOLUTION
TUBERCULOSIS
Tuberculosis (Mycobacterium tuberculosis)
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