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Real-time PCR Assay for Detection of Pneumococcal DNA and Diagnosis of Pneumococcal Disease
Case ID:
TAB-2641
Web Published:
12/6/2022
Description:
CDC scientists have developed a real-time PCR assay for diagnosing pneumococcal disease using amplification of the bacterial gene encoding pneumococcal surface adhesin A (PsaA). Pneumococcal isolation and identification is often complicated by 1) antimicrobial suppression of growth in culture and 2) contamination by normal flora alpha-streptococci. Further, pneumococcal detection by culture and serological methods can be time-consuming, relatively expensive, laborious and, ultimately, indeterminate. Sensitive and specific assays that can be completed quickly in the clinical laboratory are essential for early diagnosis and effective therapy. This RT-PCR assay provides a tool for quick and accurate diagnosis by physicians and health care technicians and may be useful in evaluating the efficacy of novel pneumococcal vaccines and therapeutics.
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Direct Link:
https://nih.technologypublisher.com/tech/Real-time_PCR_Assay_for_Detection_of _Pneumococcal_DNA_and_Diagnosis_of_Pneumococcal_Disease
Category(s):
Collaboration
Licensing
Infectious Disease
Diagnostics
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For Information, Contact:
Jonathan Motley
Fellow
NIH Technology Transfer
jonathan.motley@nih.gov
Inventors:
Edwin Ades
George Carlone
Karen McCaustland
Maria Da Gloria Carvalho
Jacquelyn Sampson
Keywords:
assay
CDC Docket Import
CDC Docket Import CDC Prosecuting
DA3XXX
DAXXXX
Detection
Development
Diagnosis
Disease
DNA
DXXXXX
PCR
PNEUMOCOCCAL
REAL-TIME
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