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High Level Expression and Purification of Untagged and Histidine-tagged Human Immunodeficiency Virus Type-1 Reverse Transcriptase

Human immunodeficiency virus type-1 reverse transcriptase (HIV-1 RT) gene encodes 560 amino acids. In the virus, however, HIV-1 RT occurs as a dimer of two related polypeptides, p66 and p51 subunits at a molar ratio of 1:1. The p51 subunit is derived from a C-terminal proteolytic cleavage of the p66 subunit. This invention describes a simplified protocol...

Published: 7/25/2024 | Inventor(s): Rajendra Prasad, Ester Hou, Samuel Wilson

Keywords(s): BBXXXX, DDXXXX, DEXXXX, DXXXXX, Expression, High, Histidine-tagged, Human, Hyper IgM syndrome, Immunodeficiency, Immunodeficiency 2, Immunodeficiency 4, Immunodeficiency-3, Level, purification, Reverse, Severe combined immunodeficiency, x-linked, TRANSCRIPTASE, TYPE-1, Untagged, virus, Wiskott Aldrich syndrome

Category(s): Collaboration Sought > Licensing, Application > Research Materials, TherapeuticArea > Infectious Disease