HOME
SEARCH
RSS FEED
SUBSCRIBE
An In-Vitro Cell System Useful For Identification of RORgamma Antagonists
Case ID:
TAB-2295
Web Published:
12/6/2022
Description:
The retinoid-related orphan receptors alpha, beta and gamma (RORalpha, beta and gamma , also referred to as NR1F1, 2 and 3, respectively) comprise a distinct subfamily of nuclear receptors. Study of ROR-deficient mice has implicated RORs in the regulation of a number of biological processes and revealed potential roles for these proteins in several pathologies. NIH investigators have developed an in-vitro system using CHO cells stably expressing a TET-On expression vector regulating RORgamma and a RORE-Luciferase reporter. This system allows inducible expression of RORgamma upon addition of doxycycline. Upon its induction RORgamma binds to the RORE in the luciferase reporter plasmid and induces luciferase. This system can be used to identify RORgamma antagonists. This system has been tested successfully in 1536-well plate high throughput analysis.
Patent Information:
Title
App Type
Country
Serial No.
Patent No.
File Date
Issued Date
Expire Date
Patent Status
Direct Link:
https://nih.technologypublisher.com/tech/An_In-Vitro_Cell_System_Useful_For_I dentification_of_RORgamma_Antagonists
Category(s):
Collaboration
Research Materials
Diagnostics
Therapeutics
Bookmark this page
Download as PDF
For Information, Contact:
Vidita Choudhry
Technology Development Specialist
NIH Technology Transfer
301-594-4095
vidita.choudhry@nih.gov
Inventors:
Yukimasa Takeda
Anton Jetten
Keywords:
AC5XXX
ACXXXX
AXXXXX
Cell
Cells
CHO
Co-expressing
DEVELOPED
Expression
IDXXXX
IXXXXX
Reporter.
RORC
RORE5-Luciferase
RORgamma
RXXXXX
System
TET-ON
Vector
Vitro
Home
|
Search
|
RSS
|
Subscribe
© 2024. All Rights Reserved. Powered by
Inteum