Argon to Improve Bioanalyte Stability in Fixed Tissue Specimens

Abstract:

The degradation of archival surgical and biospecimen limits the utility of many biomarkers that may have prognostic or predictive significance in guiding a patient’s therapy. Previous methods at preventing the degradation of RNA and proteins in formalin fixed, paraffin embedded (FFPE) tissue blocks & slides have no protective benefit. 

Researchers at the National Institutes of Health (NIH) and American Air Liquide have demonstrated that dissolved reactive gasses entrapped in the fixative and solutions used in tissue preparation contribute to this degradation. Removal of these reactive gasses, and replacement with argon, improves biomolecule stability.  The primary evaluation was based on measurements of RNA stability, however the investigator’s work demonstrates the relationships between RNA, protein, and DNA quality. 

Competitive Advantages:

• The addition of argon to FFPE procedures has been shown to improve RNA integrity by over 40% over standard FFPE procedures
• The use of the new argon FFPE procedure has been demonstrated to improve protein integrity in tissue fixed with FFPE compared with non-argon FFPE procedures

Commercial Applications:

• This novel technology may be used to generate new argon-containing fixatives to improve specimen quality without affecting traditional histopathology methods
• The novel technology includes the fixation of tissue in solutions that have been sparged with argon, as well as technology for the impregnation of tissue with solutions sparged with argon