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Ultra-sensitive Diagnostic Detects fg/mL-pg/mL Pathogen/Disease Protein by Visual Color Change
Case ID:
TAB-2896
Web Published:
12/6/2022
Description:
This technology is an ultra-sensitive colorimetric assay, based on an enzyme-catalyzed gold nanoparticle growth process, for detection of disease-associated proteins (biomarkers) and disease diagnosis. Current detection methods, such as ELISA immunoassays, measure concentrations above 0.1 ng/mL in a sample. PCR, although more sensitive than ELISA, requires expensive and specialized equipment and reagents, skilled labor, and complex analysis techniques. This assay detects fg/mL to pg/mL concentrations, allowing detection and diagnosis in the earliest stage of disease or infection. A simple to read colorless-to-red change of gold nanoparticle is read with the naked eye, without the need for advanced instruments. This assay can be performed in a standard ELISA plate. Prototype, proof of concept tests using this platform have been designed for enterovirus 71 (EV71) and prostate specific antigen (PSA). The limit of detection (LOD) for a PSA prototype exceeded the commercial ELISA by more than four orders of magnitude. This assay may be particularly well suited for field use/point-of-care detection of infections and early stage disease.
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Direct Link:
https://nih.technologypublisher.com/tech?title=Ultra-sensitive_Diagnostic_Det ects_fg%2fmL-pg%2fmL_Pathogen%2fDisease_Protein_by_Visual_Color_Change
Category(s):
Collaboration
Medical Devices
Research Equipment
Diagnostics
Non-Medical Devices
Research Materials
Infectious Disease
Oncology
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For Information, Contact:
Michael Shmilovich
Senior Licensing And Patenting Manager
NIH Technology Transfer
301-435-5019
shmilovm@nih.gov
Inventors:
Dingbin Liu
Xiaoyuan (Shawn) Chen
Keywords:
assay
AuNPbased
AuNP-based
Biomarkers
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COLORIMETRIC
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Detection
GOLD
Nanoparticle
Ultrasensitive
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